The ViraStripe® is an immunoblot based on an enzyme-immunoassay in the line/stripe format, carrying highly purified, specific antigens. It is suited for the qualitative detection of antibodies against specific antigens in human serum.
During the serum incubation step specific antibodies bind to the immobilised antigens on the test strip. During the conjugate reaction, AP-conjugate binds to the antigen-antibody complex. The alkaline phosphatase converts the chromogen/substrate and thus, stains the antigen-antibody complex on the test strip purple. The washing procedures following serum, conjugate and chromogen/substrate incubation steps remove unbound antibodies and reagents.
The green separation line divides the test strip into a control section and an analytical section. The control section contains the negative control band, the serum control, three conjugate controls (IgG, IgA, IgM) and the cut off control.