Hepcidin HS ELISA

A high sensitive enzyme immunoassay for the quantitative measurement of Hepcidin-25 in serum or plasma (EDTA-, heparin- or citrate plasma).
The Hepcidin 25 (bioactive) HS ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the principle of competitive binding. The microtiter wells are coated with a monoclonal (mouse) antibody directed towards an antigenic site of the Hepcidin-25 molecule. Endogenous Hepcidin-25 of a patient sample competes with a Hepcidin-25-biotin conjugate (Enzyme Conjugate) for binding to the coated antibody. After incubation the microtiter plate is washed to stop the competition reaction. In the following incubation the bound biotin molecules are detected with streptavidin peroxidase (Enzyme Complex). After incubation the plate is washed the second time. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Hepcidin-25 in the patient sample.

Product

Cat. nr:

Number of tests: 96 Wells

Range: 0.153 – 81 ng/mL

Incubation Time(s):  60 / 30 / 20 min

More information

Bio-Type  Serum/Plasma

Method  ELISA

Sample Volume 20 µL

Storage Conditions 2°C – 8°C

Contact

    Institute or Company
    Name
    Email
    Phonenumber
    Message or Question