ViraChip® Immunoblots

The ViraChip® is an immunoblot based on an enzyme-immunoassay in a microarray format, carrying highly purified specific antigens bound to nitrocellulose at defined positions. It is suited for the qualitative detection of antibodies against specific antigens in human serum.

One microarray is fixed at the bottom of each well in a standard microtiter plate (MTP). The single breakable wells are stored in a holding frame with 96 positions. During the serum incubation step antigen-specific antibodies bind to the immobilised antigens, herein after referred to as spots, on the microarray. During the conjugate reaction, the AP-conjugate binds to the antigen-antibody complex. The alkaline phosphatase converts the chromogen/substrate and thus, stains the antigen-antibody complex on the microarray purple. The washing procedures following serum, conjugate and chromogen/substrate incubation steps remove unbound antibodies and reagents.

The control spots include serum controls, conjugate controls, calibrator controls and a negative control.

Fully automated procedure on ELISA processors

Automated evaluation with the ViraChip® Software

 

 

Available ViraChip® Tests

Infectious Diagnostics

  • Bordetella pertussis ViraChip IgG, IgA
  • Borrelia ViraChip IgG, IgM
  • Yersinia ViraChip IgG, IgA, IgM
  • Helicobacter ViraChip IgG, IgA
  • EBV ViraChip IgG, IgM
  • HEV ViraChip IgG, IgM
  • Parvovirus ViraChip IgG, IgM
  • Treponema ViraChip IgG, IgM
  • Arbovirus ViraChip IgG, IgM

 

Autoimmune Diagnostics

  • ANA ViraChip IgG
  • Liver ViraChip IgG

ViraChip Software

The ViraChip® software performs as a central interface through the entire process of sample testing. It manages both the electronic path of the data, as well as the processing and measurement of ViraChip® tests in the microtiter plate. Subsequently, every single ViraChip® is evaluated and validated via an intuitive graphical software solution.

Central Interface
for direct communication with processor and camera

Bidirectional connection to LIS
Safe data import and export

Automated calculation
of up to 96 ViraChip® test results in a few seconds

Standardised evaluation and interpretation
with customised print outs

 

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