This in vitro diagnostic kit is intended for detection of TPMT*2, TPMT*3A, TPMT*3B and TPMT*3C in TPMT gene in human genomic DNA by realtime PCR method (allelic discrimination). The kit allows the determination of haplotypes TPMT*2, TPMT*3A, TPMT*3B and TPMT*3C in homozygous or heterozygous form. The kit does not distinguish genotype *1/*3A (two mutations in cis form) from 3B*/*3C (the incidence of the composite heterozygote *3B/3C* is unlikely in Caucasians).