Fluorescence detection is accomplished using FAM, HEX and Cy5 filters. 2019-nCoV is detected by two regions of the virus in two separate reactions: E gene is used for screening purpose, where 2019-nCoV and also the closely related coronaviruses are detected, and the orf1ab target region is used to discriminate 2019-nCoV specifically. In PCR Master Mix, 2019-nCoV target region is amplified and fluorescence detection is accomplished using FAM filter, 2019-nCoV screening region (E gene) is amplified and fluorescence detection is accomplished using the Cy5 filter.

An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with HEX filter. The internal control can be added either during RNA extraction or PCR step.